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KMID : 0525720170220030139
Journal of Chitin and Chitosan
2017 Volume.22 No. 3 p.139 ~ p.148
Antifungal Activity by Chitosan-Alginate Complex Beads with Plant Extracts and Bacillus cereus MP-310
Kang Ha-Rin

Song Yong-Su
Seo Dong-Jun
Jung Woo-Jin
Abstract
To investigate the antifungal activity of chitosan-alginate complex beads (CABs), CABs combined with Cinnamon extract, Hinoki cypress oil, and Bacillus cereus MP-310 were used as an antifungal agents for four phytopathogens (Rhizoctonia solani AG-1, R. solani AG-2-1, Fusarium oxysporum and Botrytis cinerea). Antifungal activity was measured on four phytopathogens with four different treatments of chitosan-alginate complex beads on pH 3.6: Chitosan-alginate complex beads (CAB), chitosan- Cinnamon extracts-alginate beads (CCAB), chitosan-Hinoki cypress oil-alginate beads (CHAB), and chitosan-B. cereus MP-310 medium-alginate beads (CMAB). Mixtures of CABs combined with Cinnamon extracts, Hinoki cypress oil, and B. cereus medium inhibited mycelial growth of four phytopathogenic fungi on PDA plates. In particular, twenty CABs combined with Cinnamon extract and B. cereus MP-310 medium inhibited strongly by 80% and 79.2% for mycelial growth of R. solani AG-1, respectively, at 2 days after incubation on PDA plates. Antifungal activity was measured with four Type treatments of CABs on pH 5.0: Twenty CABs combined with Cinnamon extracts and B. cereus MP-310 medium inhibited strongly by 29.6% and 32.6% for mycelial growth of R. solani AG-1, respectively, at 2 days after incubation on PDA plates. Relatively, CABs adjusted as pH 3.6 inhibited strongly the mycelial growth of R. solani AG-1. These results suggest that the combination of CABs (CABs- Cinnamon, CABs-Hinoki cypress oil, and CABs-B. cereus MP-310) has a great potential as an alternative to synthetic fungicides for the control of phytopathogens.
KEYWORD
Chitosan-alginate complex beads, Cinnamon extracts, Hinoki cypress oil, Bacillus cereus, Antifungal activity
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